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產品名稱：重組大鼠干細胞因子蛋白
產品型號：rRtSCF
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組大鼠干細胞因子蛋白與其它公司提供的重組蛋白不同，rRtSCF蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠干細胞因子蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組大鼠干細胞因子蛋白

Synonyms Hematopoietic growth factor KL、MGF

Species Rat

Accession P21581

GeneID 60427

Source Escherichia coli.

Molecular Weight 重組大鼠干細胞因子蛋白Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組大鼠干細胞因子蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine MC/9-2 mast cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rRtSCF as determined by LAL method.

Reconstitution 重組大鼠干細胞因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠干細胞因子蛋白

Reference 1. Ronnstrand L. 2004. Cell Mol Life Sci. 61:2535-48.
2. Anderson DM, Williams DE, Tushinski R, et al. 1991. Cell Growth Differ. 2:373-8.
3. Brannan CI, Lyman SD, Williams DE, et al. 1991. Proc Natl Acad Sci U S A. 88:4671-4.
4. Okayama Y, Kawakami T. 2006. Immunol Res. 34:97-115.

Background Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. In addition, it also promotes mast cell adhesion, migration, proliferation, and survival 3. Rat SCF shares 75 % - 90 % a.a. sequence identity with canine, feline, mouse, and human SCF. Furthermore, rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells.

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Synonyms	Hematopoietic growth factor KL、MGF
Species	Rat
Accession	P21581
GeneID	60427
Source	Escherichia coli.
Molecular Weight	重組大鼠干細胞因子蛋白Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組大鼠干細胞因子蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine MC/9-2 mast cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rRtSCF as determined by LAL method.
Reconstitution	重組大鼠干細胞因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠干細胞因子蛋白
Reference	1. Ronnstrand L. 2004. Cell Mol Life Sci. 61:2535-48. 2. Anderson DM, Williams DE, Tushinski R, et al. 1991. Cell Growth Differ. 2:373-8. 3. Brannan CI, Lyman SD, Williams DE, et al. 1991. Proc Natl Acad Sci U S A. 88:4671-4. 4. Okayama Y, Kawakami T. 2006. Immunol Res. 34:97-115.
Background	Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. In addition, it also promotes mast cell adhesion, migration, proliferation, and survival 3. Rat SCF shares 75 % - 90 % a.a. sequence identity with canine, feline, mouse, and human SCF. Furthermore, rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells.

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產品名稱：重組大鼠干細胞因子蛋白